Table 1 Development of luminescence syncytium induction assay
methods | reporter cell lines | identification | Non-specific background | BLV-infected cells | BLV particle | approved for milk sample | references |
|---|---|---|---|---|---|---|---|
SIA | CC81, F81 | Giemsa staining | high | + | + | – | |
LuSIA 1st. Gen. | CC81-BLU3G | auto-fluorescence | high | ++ | ++ | – | [23] |
LuSIA 2nd. Gen. | CC81-BLU3L | luciferase assay | low | ++ | ++ | NT | [24] |
LuSIA 2nd. Gen. | CC81-GREMG | auto-fluorescence | low | ++ | ++ | + | |
LuSIA 3rd. Gen. | CC81-GREMG-CAT1 | auto-fluorescence | medium | +++ | +++ | NT | this report |