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Fig. 3 | Virology Journal

Fig. 3

From: Overexpression of bovine leukemia virus receptor SLC7A1/CAT1 enhances cellular susceptibility to BLV infection on luminescence syncytium induction assay (LuSIA)

Fig. 3

Comparison of detection of cell-free infection by LuSIAs using CC81-GREMG-CAT1 and CC81-GREMG. a) CC81-GREMG-CAT1 and CC81-GREMG cells (4 × 105 cells/well) were cultured with or without culture supernatant (containing 94 ng/well of BLV p24 protein) collected from FLK-BLV cells. The cells were cultured including 20 ng/mL of Hoechst 33342 (Sigma-Aldrich) and fluorescent syncytia are observed daily with an EVOS2 fluorescence microscope and analyzed with HCS studio software. The scale bar (white bars) indicate 275 μm. b Fluorescent syncytia were detected by LuSIAs using CC81-GREMG-CAT1 and CC81-GREMG at 3 days post-cultivation. The results indicate the mean and standard deviation of three independent experiments

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