Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: H2 influenza A virus is not pathogenic in Tmprss2 knock-out mice

Fig. 1

TMPRSS2 cleaves H2-HA. Human embryonic kidney 293 T cells were cotransfected with plasmids encoding H2-HA and plasmids encoding TMPRSS2 of murine origin or empty plasmid (Mock) as negative control. At 48 h post transfection, cells were harvested and treated with either PBS or TPCK trypsin followed by analysis of HA expression by immunoblot, using antiserum raised against H2-HA. Detection of β-actin (ACTB) served as loading control. The results were confirmed in an independent experiment. The black arrow indicates uncleaved HA0 (HA0), the grey arrow indicates cleaved HA1 (HA1)

Back to article page
\