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Fig. 3 | Virology Journal

Fig. 3

From: Identification of novel human adenovirus candidates using the coxsackievirus and adenovirus receptor for cell entry

Fig. 3

Infection efficiencies for HAdV5, 3, 14, 50 and 69 on CHO-CAR and CHO-K1 control cells and quantification of HAdV50 and HAdV69 vector genomes over time in CHO-CAR cells. Cells were infected with HAdV3 (a), HAdV14 (b), HAdV5 (c) and HAdV50 (d) and HAdV69 (e) at 100 viral particle numbers per cell (vpc) and luciferase expression levels were measured 26 h post-infection. As positive control, HAdV5 was applied and uninfected cells were measured referring to the negative control. For each virus, experiments were performed in biological and technical triplicates. (f) Quantification of HAdV50 and HAdV69 vector genomes in CHO-CAR cells after 4 h, 1 day, 2 days and 3 days after infection with 100 vpc using a digital droplet PCR approach. For statistical analyses a student’s t-test was performed. Displayed are means + standard deviation. * p-values ≤ 0.05, ** ≤ 0.005

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