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Fig. 6 | Virology Journal

Fig. 6

From: Circular RNA hsa_circ_0004812 impairs IFN-induced immune response by sponging miR-1287-5p to regulate FSTL1 in chronic hepatitis B

Fig. 6

Circ_0004812/miR-1287-5p/FSTL1 axis regulated HBV-induced immune suppression. a-f pHBV1.3 was used to transfect the Huh7 cells. After that, the pHBV1.3-transfected Huh7 cells were co-transfected with si-NC plus NC-inhibitor, si-Circ_0004812#1 plus NC-inhibitor, or si-circ_0004812#1 plus miR-1287-5p-inhibitor. Next, (b-c) the mRNA and protein levels of FSTL1 were determined using qRT-RCR and Western blotting, respectively. c HBV DNA copies were determined using qRT-PCR. d Levels of HBsAg and HBeAg were measured using specific ELISAs and the ration of HBsAg to HBeAg was calculated. e-f The mRNA and protein levels of IFN-α and IFN-β were determined using qRT-PCR and Western blotting, respectively. The ratio of IFN-α to IFN-β were calculated. Data were shown as mean ± S.D. *P < 0.05, **P < 0.01

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