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Fig. 4 | Virology Journal

Fig. 4

From: Circular RNA hsa_circ_0004812 impairs IFN-induced immune response by sponging miR-1287-5p to regulate FSTL1 in chronic hepatitis B

Fig. 4

Circ_0004812 promoted the expression of FSTL1 through inhibition of miR-1287-5p. a The binding sites between miR-1287-5p, FSTL1 3’UTR-WT, and FSTL1 3’UTR-MT were predicted and showed. b-c The relative luciferase activities were determined in the pHBV-transfected Huh7 and HepG2.2.15 cells that were co-transfected with FSTL1 3’UTR-WT plus miR-NC, FSTL1 3’UTR-WT plus miR-1287-5p, FSTL1 3’UTR-WT plus miR-NC, or FSTL1 3’UTR-MT plus miR-1287-5p. d-e RNA pull-down assays were used to detect the relative enrichment of RNA including circ_0004812 and FSTL1 in pHBV-transfected Huh7 and HepG2.2.15 cells. f-g The relative mRNA and protein levels of FSTL1 were determined using qRT-PCR and Western blotting, respectively, in pHBV-transfected Huh7 and HepG2.2.15 cells that were co-transfected with miR-1287-5p mimics and miR-1287-5p inhibitor or the negative controls. h-j The relative mRNA and protein levels of FSTL1 were determined using qRT-PCR and Western blotting, respectively, in pHBV-transfected Huh7 and HepG2.2.15 cells that were co-transfected with Circ_0004812 siRNAs including si-Circ_0004812#1 and si-Circ_0004812#2) or the negative control siRNA (si-NC), or plasmids containing sequence of Circ_0004812 or empty vector (VEC). Data were shown as mean ± S.D. *P < 0.05, **P < 0.01, and ns indicates not significant

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