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Fig. 3 | Virology Journal

Fig. 3

From: Circular RNA hsa_circ_0004812 impairs IFN-induced immune response by sponging miR-1287-5p to regulate FSTL1 in chronic hepatitis B

Fig. 3

Circ_0004812 regulated the levels of miR-1287-5p. a-b qRT-PCR was applied to evaluate the abundance of circ_0004812 in the cytoplasm and nuclei in HepG2.2.15 cells and Huh7 cells that were transfected pHBV1.3. Two internal controls including cytoplasmic control (GADPH) and nuclear control (U6) were used. c The binding sites between miR-1287-5p and Circ_0004812 wild-type (circ_0004812-WT) and mutant (circ_0004812-MT) were shown. d-e Relative luciferase activities were determined in pHBV-transfected Huh7 or HepG2.2.15 cells co-transfected with circ_0004812-WT, circ_0004812-MT or psiCHECK2 empty vector and miR-1287-5p or miR-NC. f The relative expression of miR-1287-5p were determined using qRT-PCR in pHBV-transfected Huh7 and HepG2.2.15 cells that were co-transfected with negative control siRNA (si-NC), circ_0004812 siRNAs including (si-circ_0004812#1, si-Circ_0004812#2). Besides, (g) the relative expression of miR-1287-5p were also determined using qRT-PCR in pHBV-transfected Huh7 and HepG2.2.15 cells that were co-transfected with circ_0004812 overexpression plasmid (circ_0004812-OE) or empty vector (VEC). Data were shown as mean ± S.D. *P < 0.05, **P < 0.01, and ns indicates not significant

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