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Fig. 2 | Virology Journal

Fig. 2

From: Circular RNA hsa_circ_0004812 impairs IFN-induced immune response by sponging miR-1287-5p to regulate FSTL1 in chronic hepatitis B

Fig. 2

Knockdown of circ_0004812 affected anti-HBV efficiency by regulating the levels of IFNs. a-f pHBV1.3 was used to transfect the Huh7 cells. After that, the Huh7 cells were co-transfected with negative control siRNA (si-NC), circ_0004812 siRNAs including (si-circ_0004812#1, si-circ_0004812#2). a The relative expression of circ_0004812 were measured using qRT-PCR. b-c Levels of HBsAg and HBeAg were measured using specific ELISAs. d HBV DNA copies were determined using qRT-PCR. (E-F) The mRNA and protein levels of IFN-α and IFN-β were determined using qRT-PCR and Western blotting, respectively. Data were shown as mean ± S.D. *P < 0.05, **P < 0.01

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