From: Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea
Virus | Target gene | Primer sequences | PCR condition | Product size (bp) |
---|---|---|---|---|
parvovirus | NS | PV-CLC-F1 5′-ACGAAGGTAAGAATGAGG-3′ PV-CLC-R1 5′-GGAGATTGATTGGGGATG-3’ | PCR 95 °C, 5 min - 30 cycles(94 °C, 30s, 55 °C, 1 min, 72 °C, 1 min) - 72 °C, 5 min | 364 |
picornavirus | NS | Picorna-F3 5’-TACCCGAGAAAACGACCC-3′ Picorna-R3 5′-ACACCTCAGCTACAAGAA-3’ | Multiplex RT-PCR 50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 50 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min | 504 |
astrovirus | ORF1a | Astro-F1 5’-ATTCCAGTTCAGCTCTTC-3′ Astro-R1 5′-TCCTGTTGGTAAGAGAGT-3’ | 138 | |
calicivirus | VP1 | Calici-CLC-F3 5’-AGAAGCGTGACTACATGGA-3′ Calici-CLC-R1 5′-TTGTGTTGTTTGTGGGGT-3’ | 1247 | |
rotavirus A | VP6 | RotaA-CLC-F2 5’-CTCCTCAATCTAATGCACT-3′ RotaA-CLC-R2 5′-CCGAACCATTATTTAGCCA-3’ | Multiplex RT-PCR 50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 60 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min | 230 |
rotavirus Da | VP6 | RotaD-F 5’-GGAGGCGCTGTCTTCAATTGCG-3′ RotaD-R 5′-TGGCCAATAGTGTGTGGCAGCT-3’ | 742 | |
rotavirus F | VP6 | RotaF-CLC-F2 5’-GTGGAGTCGGAAATATGG-3′ RotaF-CLC-R2 5′-CAGGTACAACATAAGGATTC-3’ | 330 |