Skip to main content

Table 1 Primers and PCR instrumental conditions for detecting enteric viruses

From: Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

VirusTarget genePrimer sequencesPCR conditionProduct size (bp)
parvovirusNSPV-CLC-F1 5′-ACGAAGGTAAGAATGAGG-3′
PV-CLC-R1 5′-GGAGATTGATTGGGGATG-3’
PCR
95 °C, 5 min - 30 cycles(94 °C, 30s, 55 °C, 1 min, 72 °C, 1 min) - 72 °C, 5 min
364
picornavirusNSPicorna-F3 5’-TACCCGAGAAAACGACCC-3′
Picorna-R3 5′-ACACCTCAGCTACAAGAA-3’
Multiplex RT-PCR
50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 50 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min
504
astrovirusORF1aAstro-F1 5’-ATTCCAGTTCAGCTCTTC-3′
Astro-R1 5′-TCCTGTTGGTAAGAGAGT-3’
138
calicivirusVP1Calici-CLC-F3 5’-AGAAGCGTGACTACATGGA-3′
Calici-CLC-R1 5′-TTGTGTTGTTTGTGGGGT-3’
1247
rotavirus AVP6RotaA-CLC-F2 5’-CTCCTCAATCTAATGCACT-3′
RotaA-CLC-R2 5′-CCGAACCATTATTTAGCCA-3’
Multiplex RT-PCR
50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 60 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min
230
rotavirus DaVP6RotaD-F 5’-GGAGGCGCTGTCTTCAATTGCG-3′
RotaD-R 5′-TGGCCAATAGTGTGTGGCAGCT-3’
742
rotavirus FVP6RotaF-CLC-F2 5’-GTGGAGTCGGAAATATGG-3′
RotaF-CLC-R2 5′-CAGGTACAACATAAGGATTC-3’
330
  1. aprimers for detecting rotavirus D were used according to the reference [14]