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Table 1 Primers and PCR instrumental conditions for detecting enteric viruses

From: Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Virus

Target gene

Primer sequences

PCR condition

Product size (bp)

parvovirus

NS

PV-CLC-F1 5′-ACGAAGGTAAGAATGAGG-3′

PV-CLC-R1 5′-GGAGATTGATTGGGGATG-3’

PCR

95 °C, 5 min - 30 cycles(94 °C, 30s, 55 °C, 1 min, 72 °C, 1 min) - 72 °C, 5 min

364

picornavirus

NS

Picorna-F3 5’-TACCCGAGAAAACGACCC-3′

Picorna-R3 5′-ACACCTCAGCTACAAGAA-3’

Multiplex RT-PCR

50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 50 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min

504

astrovirus

ORF1a

Astro-F1 5’-ATTCCAGTTCAGCTCTTC-3′

Astro-R1 5′-TCCTGTTGGTAAGAGAGT-3’

138

calicivirus

VP1

Calici-CLC-F3 5’-AGAAGCGTGACTACATGGA-3′

Calici-CLC-R1 5′-TTGTGTTGTTTGTGGGGT-3’

1247

rotavirus A

VP6

RotaA-CLC-F2 5’-CTCCTCAATCTAATGCACT-3′

RotaA-CLC-R2 5′-CCGAACCATTATTTAGCCA-3’

Multiplex RT-PCR

50 °C, 30 min - 94 °C, 2 min - 40 cycles(94 °C, 30s, 60 °C, 30s, 72 °C, 1 min) - 72 °C, 5 min

230

rotavirus Da

VP6

RotaD-F 5’-GGAGGCGCTGTCTTCAATTGCG-3′

RotaD-R 5′-TGGCCAATAGTGTGTGGCAGCT-3’

742

rotavirus F

VP6

RotaF-CLC-F2 5’-GTGGAGTCGGAAATATGG-3′

RotaF-CLC-R2 5′-CAGGTACAACATAAGGATTC-3’

330

  1. aprimers for detecting rotavirus D were used according to the reference [14]