Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

Baba, Satoshi; Taguchi, Ayumi; Kawata, Akira; Hara, Konan; Eguchi, Satoko; Mori, Mayuyo; Adachi, Katsuyuki; Mori, Seiichiro; Iwata, Takashi; Mitsuhashi, Akira; Maeda, Daichi; Komatsu, Atsushi; Nagamatsu, Takeshi; Oda, Katsutoshi; Kukimoto, Iwao; Osuga, Yutaka; Fujii, Tomoyuki; Kawana, Kei

doi:10.1186/s12985-020-01306-0

Virology Journal

Table 1 Primer pairs used for qRT-PCR

From: Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

Target	Direction	Sequence	Product size (bp)	Genome position
GAPDH	Forward	GAAAGGTGAAGGTCGGAGTC	227
GAPDH	Reverse	GAAGATGGTGATGGGATTTC	227
HPV 16 E6	Forward	AGCGACCCAGAAAGTTACCAC	260	123–143
HPV 16 E6	Reverse	GTTGTATTGCTGTTCTAATGTTG		382–360
HPV 16 E6*	Forward	AGCGACCCAGAAAGTTACCAC	114	123–143
HPV 16 E6*	Reverse	TTAATACACCTCACGTCGC		418–409 + 226–217
HPV 16 E1^4	Forward	CCTGCAGCAGCAACGAAGTATC	218	874–880 + 3358–3372
HPV 16 E1^4	Reverse	TTGGTCGCTGGATAGTCGTC		3479–3460
HPV 16 L1	Forward	GTCTCTTTGGCTGCCTAGTG	89	5641–5660
HPV 16 L1	Reverse	TGCGTGCAACATATTCATCCG		5729–5709
HPV 18 E6	Forward	AACACGGCGACCCTACAAG	248	125–143
HPV 18 E6	Reverse	ATGTGTCTCCATACACAGAGTC		372–351
HPV 18 E6*	Forward	AACACGGCGACCCTACAAG	120	125–143
HPV 18 E6*	Reverse	ACCGCAGGCACCTCTGTAAG		426–416 + 233–225
HPV 18 E1^4	Forward	GATCCAGAAGTACCAGTGAC	194	920–929 + 3434–3443
HPV 18 E1^4	Reverse	GAGAAGTGGGTTGACAGGTC		3617–3598
HPV 18 L1	Forward	TCCTTCTGTGGCAAGAGTTGT	123	5657–5677
HPV 18 L1	Reverse	CCACCTGCAGGAACCCTAAAA		5779–5759
HPV 52 E6	Forward	TTTGAGGATCCAGCAACAC	197	105–123
HPV 52 E6	Reverse	TAGGCACATAATACACACGCC		302–282
HPV 52 E6*	Forward	TTTGAGGATCCAGCAACAC	128	105–123
HPV 52 E6*	Reverse	GACAAATTATACATCTCTCTTCG		510–502 + 216–224
HPV 52 E1^4	Forward	AGGACCCTGAAGTAACGAAG	150	868–879 + 3345–3352
HPV 52 E1^4	Reverse	CTGGAGTCTGTGACGTCTGG		3482–3463
HPV 52 L1	Forward	ACTGTGTACCTGCCTCCTGTA	72	5670–5690
HPV 52 L1	Reverse	GATGCTTGTGCGAGACACAT		5741–5722
HPV 58 E6	Forward	GAAACCACGGACATTGCATG	254	130–149
HPV 58 E6	Reverse	GTGTTTGTTCTAATGTGTCTCC		383–362
HPV 58 E6*	Forward	GAAACCACGGACATTGCATG	109	130–149
HPV 58 E6*	Reverse	CAAATAATACATCTCAGATCGC		515–510 + 232–223
HPV 58 E1^4	Forward	GACCCTGAAGTGATCAAATATC	127	889–898 + 3358–3372
HPV 58 E1^4	Reverse	GTGTTGTCTCTGGAGTCTGG		3471–3452
HPV 58 L1	Forward	CCTCCTGTGCCTGTGTCTAA	104	5682–5700
HPV 58 L1	Reverse	GGATTGCCAACAGCCAAAAGT		5785–5765

Primer information, such as sequence, product size, and genome position of the primer pairs was summarized

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ISSN: 1743-422X

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