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Table 1 Sequence of the primers used in this study

From: Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus

Names

Primer sequences (5′ → 3′)

Positiona

SA

F: TACCCTCGAGGGATCCATGAAGTCTTTAACCTACTTCTGGTTGT

R: GCTTGAATTCGGATCCTTAAGCACAACCTCCACTGTTGTAACA

1–609

SB

F: TACCCTCGAGGGATCCATGACATGGGATAATGATCGTGTCACT

R: GCTTGAATTCGGATCCTTATAAGTGAGGATCTGAGGAATTACTGC

499–1071

SC

F:TACCCTCGAGGGATCCATGAATATTAATGACACCTCTCTCATTCTTG

R: GCTTGAATTCGGATCCTTAAAACCCATTGATAGTAGTGTCAGATG

964–1617

SD

F: TACCCTCGAGGGATCCATGCCATCATTTAATGATCATTCTTTTGTT

R: GCTTGAATTCGGATCCTTACACTATATCATCATCAACATATGCAGC

1513–2142

SE

F: TACCCTCGAGGGATCCATGAATTCTAGCTTTTTGGCAGGTG

R: GCTTGAATTCGGATCCTTAACTAAAGTTGGTGGGAATACTAATATTC

1999–2367

SE1

F: TACCCTCGAGGGATCCATGAATTCTAGCTTTTTGGCAGGTG

R: GCTTGAATTCGGATCCTTAATCATCAACATATGCAGCCTGCTCT

1999–2133

SE2

F: TACCCTCGAGGGATCCATGACTAGTGGTGCTGTTTATTCTGTTA

R: GCTTGAATTCGGATCCTTAGTAGAAGAAACCAGGCAACTCCCTA

2068–2208

SE3

F: TACCCTCGAGGGATCCATGGCATATGTTGATGATGATATAGTGG

R: GCTTGAATTCGGATCCTTAACTGCCAGATTTACAAACACCTATG

2119–2283

SE4

F: TACCCTCGAGGGATCCATGTCTAATTGTACAGAGCCTGTGTTGG

R: GCTTGAATTCGGATCCTTAACTAAAGTTGGTGGGAATACTAATATTC

2224–2367

SE5

F: TACCCTCGAGGGATCCATGGCATATGTTGATGATGATATAGTGG

R: GCTTGAATTCGGATCCTTAGTTAAAAGTGGAGCTAGACAAACTA

2119–2178

SE6

F: TACCCTCGAGGGATCCATGATTTCTAGTTTGTCTAGCTCCACTT

R: GCTTGAATTCGGATCCTTAATTTCTAGTTTGTCTAGCTCCACTT

2149–2208

  1. The introduced restriction enzyme sites BamH I are underlined. At 5′ terminal of each sense strand, there is a sequence of TACCCTCGAG (bold) which is as same as the ends of carrier Pcold-TF. At 3′ terminal of each reverse sense strand, there is a sequence of GCTTGAATTC (bold) complement with the ends of carrier Pcold-TF
  2. aLocation of the synthesized peptides is based on the sequence of S protein of PEDV strain HeB/TS/2016/325b (GenBank accession no. KX907110.1)