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Fig. 1 | Virology Journal

Fig. 1

From: Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus

Fig. 1

Detection of recombinant proteins’ antigenicity for polyclonal antisera (PcAbs) by indirect ELISA, western blot and IFA. a Reactivity of antisera against the recombinant S1 protein by indirect ELISA. b Western blot analysis of the recombinant S1 protein with PcAbs. The recombinant S1 protein (approximately 140 kDa) was transferred to the nitrocellulose (NC) membrane, followed by the different PcAbs (negative control, SA polyclonal antisera, SB polyclonal antisera, SC polyclonal antisera, SD polyclonal antisera and SE polyclonal antisera) as primary antibody. c Immunofluorescence analysis of SE polyclonal antisera against PEDV. Vero cells were plated in six-well plates and inoculated with PEDV (0.01 MOI). Twenty-four hours later, cells were fixed and incubated with SE polyclonal antisera or normal mouse serum (negative control), and then incubated with Alexa Fluor 488 donkey anti-mouse IgG (H + L) antibody. Scale bars: 200 μm

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