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Table 1 Real-Time PCR Primes

From: The USP18 cysteine protease promotes HBV production independent of its protease activity

Gene NameNucleotide sequence
GAPDHForward 5′-GCCTCCTGCACCACCAACTG-3′
Reverse 5′-ACGCCTGCTTCACCACCTTC-3′
USP18Forward 5′-CAGACCCTGACAATCCACCT-3′
Reverse 5′-AGCTCATACTGCCCTCCAGA-3′
HBV total DNAForward 5′-CGTTTTTGCCTTCTGACTTCTTTC-3′
Reverse 5′-ATAGGATAGGGGCATTTGGTGGTC-3′
HBV cccDNAForward 5′-TTCTCATCTGCCGGACCG-3′
Reverse 5′-CACAGCTTGGAGGCTTGAAC-3’
HBV pgRNAForward 5’-CTCAATCTCGGGAATCTCAATGT-3′
Reverse 5′-TGGATAAAACCTAGCAGGCATAAT-3’
MxAForward 5’-GTGCATTGCAGAAGGTCAGA-3′
Reverse 5′-CTGGTGATAGGCCATCAGGT-3’
OAS2Forward 5’-TCAGCGAGGCCAGTAATCTT-3′
Reverse 5′-GCAGGACATTCCAAGATGGT-3’
  1. USP18 Ubiquitin specific protease 18, HBV cccDNA hepatitis B virus covalently closed circular DNA, pgRNA hepatitis B virus pregenomic RNA, MxA myxovirus resistance protein A, OAS2 2’,5′-oligoadenylate synthetase 2