Table 1 Real-Time PCR Primes
From: The USP18 cysteine protease promotes HBV production independent of its protease activity
Gene Name | Nucleotide sequence |
|---|---|
GAPDH | Forward 5′-GCCTCCTGCACCACCAACTG-3′ Reverse 5′-ACGCCTGCTTCACCACCTTC-3′ |
USP18 | Forward 5′-CAGACCCTGACAATCCACCT-3′ Reverse 5′-AGCTCATACTGCCCTCCAGA-3′ |
HBV total DNA | Forward 5′-CGTTTTTGCCTTCTGACTTCTTTC-3′ Reverse 5′-ATAGGATAGGGGCATTTGGTGGTC-3′ |
HBV cccDNA | Forward 5′-TTCTCATCTGCCGGACCG-3′ Reverse 5′-CACAGCTTGGAGGCTTGAAC-3’ |
HBV pgRNA | Forward 5’-CTCAATCTCGGGAATCTCAATGT-3′ Reverse 5′-TGGATAAAACCTAGCAGGCATAAT-3’ |
MxA | Forward 5’-GTGCATTGCAGAAGGTCAGA-3′ Reverse 5′-CTGGTGATAGGCCATCAGGT-3’ |
OAS2 | Forward 5’-TCAGCGAGGCCAGTAATCTT-3′ Reverse 5′-GCAGGACATTCCAAGATGGT-3’ |