Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: The USP18 cysteine protease promotes HBV production independent of its protease activity

Fig. 2

Over-expression of USP18 in HepAD38 cells promoted HBV production independent of its protease activity. HepAD38 cells were transfected with USP18 plasmids as indicated. Real-time PCR was performed to quantify HBV production. Total supernatant HBV DNA (a), total intracellular HBV DNA (b), HBV cccDNA (c) and HBV pgRNA (d) 48 h post transfection with 4 μg WT-USP18 plasmid. Supernatant HBV total DNA 48 h post transfection with 4 μg WT-USP18 or 4 μg USP18-C64S plasmid, respectively (e). WT-USP18, wide type USP18; MOCK, empty plasmid. Results are presented as means ± SD (n ≥ 3). * p ≤ 0.05; ** p ≤ 0.01; ***p ≤ 0.001

Back to article page
\