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Fig. 1 | Virology Journal

Fig. 1

From: The USP18 cysteine protease promotes HBV production independent of its protease activity

Fig. 1

Over-expression of USP18 and its catalytic activity. HepAD38 cells were transfected with WT-USP18 plasmid, USP18-C64S plasmid or empty vector (MOCK) or left untreated. a: Twenty-four hours after transfection, USP18 mRNA was determined by real-time PCR (normalized by GAPDH). b: Forty-eight hours after transfection, USP18 protein expressions were analyzed by western blot (left). The relative expression levels of USP18 (normalized by GAPDH) were calculated by densitometry analysis (right). c: Cleavage of ISG15-GST fusion in vitro. USP18, ISG15/GST and WT-USP18 (or USP18-C64S) were co-transfected into Hela cells. Total intracellular protein was collected to perform Western blot. WT-USP18, wide type USP18; MOCK, empty plasmid. Results are presented as means ± SD (n ≥ 3). ** p ≤ 0.01; *** p ≤ 0.001

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