Fig. 5

Accumulation of CMV in radish (cv. Tokinashi). a Detection of CMV in inoculated cotyledons at 10 dpi and b in upper, non-inoculated leaves and root at 21 dpi by tissue immunoblotting using antiserum against CMV-Y CP. Cotyledons of 7-day-old seedlings were mechanically inoculated with chimeric RNA3s, combined with Y RNA1 and D8 RNA2. Numbers at top represent individual test plants; numbers on the left indicate leaf positions from the top of the plants. M, mock-inoculated plants. Asterisks indicate dead or senesced leaves that could not be analyzed