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Fig. 1 | Virology Journal

Fig. 1

From: The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat protein

Fig. 1

Accumulation of cucumber mosaic virus (CMV) in radish (cv. Tokinashi). a Relative accumulation levels of CMV RNA2 in inoculated cotyledons were quantified by relative RT-qPCR. b Detection of CMV in the upper, non-inoculated leaves by RNA3-targeted RT-PCR. Data are mean values from three technical replicates with three biological replicates for each cDNA; vertical bars indicate standard errors. Data were analyzed by the ∆∆Ct method followed by analysis of significant differences using Tukey test (P < 0.05 or P < 0.01). Different letters on the bars indicate statistically significant difference between the leaf samples infected with CMV-Y and CMV-D8. Data were first normalized using the GAPDH/ ACTIN2/7 genes

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