Fig. 1From: The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat proteinAccumulation of cucumber mosaic virus (CMV) in radish (cv. Tokinashi). a Relative accumulation levels of CMV RNA2 in inoculated cotyledons were quantified by relative RT-qPCR. b Detection of CMV in the upper, non-inoculated leaves by RNA3-targeted RT-PCR. Data are mean values from three technical replicates with three biological replicates for each cDNA; vertical bars indicate standard errors. Data were analyzed by the ∆∆Ct method followed by analysis of significant differences using Tukey test (P < 0.05 or P < 0.01). Different letters on the bars indicate statistically significant difference between the leaf samples infected with CMV-Y and CMV-D8. Data were first normalized using the GAPDH/ ACTIN2/7 genesBack to article page