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Table 1 Primers used for multiplex PCR of human herpesviruses in the study

From: Co-infections of human herpesviruses (CMV, HHV-6, HHV-7 and EBV) in non-transplant acute leukemia patients undergoing chemotherapy

Viruses (GenBank accession)

Primer

Primer sequence (5′-3′)

Position

Product length (bp)

HSV-1/2

(HSV-1:M10792,

HSV-2: M16321)

HSV-F

ATCCAGTACGTCTTTGTGGAGCCCAAG

HSV-1:3389

292

HSV-2:3058

HSV-R

TGAGGACAAAGTCCTGGATGTCCCTCT

HSV-1:3680

HSV-2:3349

VZV

(X04370)

VZV-F

TCCGACATGCAGTCAATTTCAACGTC

49651

161

VZV-R

GGTCGGGTAGACGCTACCACTCGTTT

49811

EBV

(NC_007605)

EBV-F

CTTAGAATGGTGGCCGGGCTGTAAAAT

153240

229

EBV-R

ATCCAGTACGTCTTTGTGGAGCCCAAG

153468

CMV

(NC_001347)

CMV-F

GCGCGTACCGTTGAAAGAAAAGCATAA

80362

131

CMV-R

TGGGCACTCGGGTCTTCATCTCTTTAC

80492

HHV6A/B

(HHV6A: NC_001664,HHV6B: AB021506)

HHV6-F

ATGCGCCATCATAATGCTCGGATACA

HHV-6A: 57837

183

HHV-6B: 58791

HHV6-R

CCCTGCATTCTTACGGAAGCAAAACG

HHV-6A: 58019

HHV-6B: 58973

HHV7

(NC_001716)

HHV7-F

GCCCGTTTTCGGAAATATTGGAGAGAT

55671

347

HHV7-R

ACGCACGAGACGCACTTTTCTTAAACA

56017

  1. Primers and targets used in the Multiplex PCR assay for the simultaneous detection of herpesviruses (HSV-1/2, VZV, EBV, CMV, HHV-6A/B, and HHV-7). Each primer pair was designed with different amplicon size to identify each product with agarose gel electrophoresis