Fig. 3
From: Characterization of HIV-1 uncoating in human microglial cell lines
Effect of CA mutations on uncoating in CHME3-TC cells. The CsA washout assay was performed with CA mutant viruses using HIV-GFP as the wildtype CA control. Line graphs shown the uncoating kinetics from a representative CsA washout assay. Errors bars denote standard error among triplicate wells. Bar graphs compare the average half-life of uncoating of CA mutant viruses and the wildtype HIV-GFP control from multiple independent experiments. Error bars denote standard error among these independent experiments. * indicates a significant difference of P < 0.05. a The N74D mutation significantly decreased the rate of uncoating among six independent experiments. b The E45A mutation significantly decreased the rate of uncoating among six independent experiments. c The compensatory mutation R132T was able to rescue the uncoating kinetics of the E45A mutation to wildtype levels in five independent experiments. d The A92E mutation did not significantly alter the rate of uncoating among seven independent experiments