Fig. 5

Detection of natural ChiVMV infection in selected field-collected tobacco leaves. a Using RT-RPA assay. b Using RT-PCR assay. Lane M, Trans2K DNA marker; lane 1, healthy tobacco leaves; lane 2, distilled water; lane 3, ChiVMV-infected tobacco plants; lanes 4–13, random field-collected tobacco samples with ChiVMV or viral-like symptoms