Table 2 Functional profile of mutants
Mutants | Avg cell surface expression (% of wt) | Avg cell fusion (% of wt) | Avg HADa (% of wt) | Avg HADb (% of wt) | Avg NAc (% of wt) | Avg NAd (% of wt) |
|---|---|---|---|---|---|---|
T167A | 93.17 ± 11.00 | 54.74 ± 3.16 | 55.81 ± 4.98 | 107.34 ± 3.70 | 98.41 ± 7.50 | 90.43 ± 5.06 |
G171A | 103.86 ± 4.92 | 104.69 ± 5.02 | 85.08 ± 1.41 | 93.69 ± 15.89 | 71.88 ± 3.93 | 87.10 ± 6.18 |
C172A | 97.29 ± 4.63 | 5.70 ± 7.48 | 4.08 ± 3.57 | – | 70.88 ± 12.64 | 87.54 ± 10.87 |
R174A | 83.46 ± 10.21 | 5.30 ± 5.20 | 2.96 ± 4.38 | – | 81.56 ± 4.19 | 90.27 ± 5.33 |
C196A | 100.17 ± 14.86 | 0.93 ± 4.25 | 6.42 ± 2.48 | – | 63.08 ± 10.70 | 86.67 ± 5.77 |
D198A | 104.70 ± 14.92 | 10.54 ± 7.24 | 4.42 ± 3.82 | – | 73.15 ± 11.07 | 96.67 ± 5.77 |
S202A | 106.20 ± 7.77 | 49.46 ± 5.30 | 44.53 ± 3.98 | 60.31 ± 6.63 | 64.50 ± 8.57 | 101.45 ± 2.51 |
R516A | 103.05 ± 4.80 | 65.26 ± 9.20 | 69.02 ± 7.45 | 68.68 ± 14.02 | 76.62 ± 1.36 | 84.42 ± 9.20 |
Y526A | 102.93 ± 8.71 | 8.38 ± 6.67 | 1.78 ± 4.29 | – | 60.25 ± 5.94 | 87.53 ± 10.87 |
E547A | 98.93 ± 6.10 | 7.06 ± 7.85 | 4.42 ± 2.84 | – | 42.04 ± 12.55 | 88.55 ± 7.93 |
Ch1 | 0.31 ± 15.13 | -e | – | – | – |  |
Ch2 | 22.52 ± 7.03 | – | – | – | – |  |
Ch3 | 102.50 ± 18.23 | 33.77 ± 2.46 | 46.96 ± 0.41 | 41.73 ± 7.76 | 63.06 ± 10.01 | 88.77 ± 7.82 |
Ch4 | 49.57 ± 4.96 | 15.89 ± 3.89 | 16.88 ± 1.33 | 23.98 ± 8.40 | 43.91 ± 8.57 | 78.99 ± 8.52 |
De1 | 7.20 ± 6.03 | – | – | – | – |  |
De2 | 5.26 ± 7.36 | – | – | – | – |  |
De3 | 17.03 ± 7.25 | – | – | – | – |  |
De4 | 10.44 ± 8.93 | – | – | – | – |  |