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Table 2 Functional profile of mutants

From: Roles of the highly conserved amino acids in the second receptor binding site of the Newcastle disease virus HN protein

MutantsAvg cell surface expression (% of wt)Avg cell fusion (% of wt)Avg HADa (% of wt)Avg HADb (% of wt)Avg NAc (% of wt)Avg NAd (% of wt)
T167A93.17 ± 11.0054.74 ± 3.1655.81 ± 4.98107.34 ± 3.7098.41 ± 7.5090.43 ± 5.06
G171A103.86 ± 4.92104.69 ± 5.0285.08 ± 1.4193.69 ± 15.8971.88 ± 3.9387.10 ± 6.18
C172A97.29 ± 4.635.70 ± 7.484.08 ± 3.5770.88 ± 12.6487.54 ± 10.87
R174A83.46 ± 10.215.30 ± 5.202.96 ± 4.3881.56 ± 4.1990.27 ± 5.33
C196A100.17 ± 14.860.93 ± 4.256.42 ± 2.4863.08 ± 10.7086.67 ± 5.77
D198A104.70 ± 14.9210.54 ± 7.244.42 ± 3.8273.15 ± 11.0796.67 ± 5.77
S202A106.20 ± 7.7749.46 ± 5.3044.53 ± 3.9860.31 ± 6.6364.50 ± 8.57101.45 ± 2.51
R516A103.05 ± 4.8065.26 ± 9.2069.02 ± 7.4568.68 ± 14.0276.62 ± 1.3684.42 ± 9.20
Y526A102.93 ± 8.718.38 ± 6.671.78 ± 4.2960.25 ± 5.9487.53 ± 10.87
E547A98.93 ± 6.107.06 ± 7.854.42 ± 2.8442.04 ± 12.5588.55 ± 7.93
Ch10.31 ± 15.13-e 
Ch222.52 ± 7.03 
Ch3102.50 ± 18.2333.77 ± 2.4646.96 ± 0.4141.73 ± 7.7663.06 ± 10.0188.77 ± 7.82
Ch449.57 ± 4.9615.89 ± 3.8916.88 ± 1.3323.98 ± 8.4043.91 ± 8.5778.99 ± 8.52
De17.20 ± 6.03 
De25.26 ± 7.36 
De317.03 ± 7.25 
De410.44 ± 8.93 
  1. The average of cell surface expression, cell fusion, HAD ability and NA activity were determined by FACS, Report Gene Method, HAD assay and NA assay, respectively. Results were expressed as mean ± SD of three independent experiments
  2. aThe results of HAD assay when the BHK-21 monolayers were treated with 1% cRBC solution in serum-free, CO2-independent medium without zanamivir
  3. bSame with the experimental conditions in a except the cRBC solution with zanamivir (2 mM)
  4. cThe results of NA assay when zanamivir was absence in the medium
  5. dSame with the experimental conditions in c except zanamivir (2 mM) was presence in the medium
  6. eNot detected