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Fig. 5 | Virology Journal

Fig. 5

From: Roles of the highly conserved amino acids in the second receptor binding site of the Newcastle disease virus HN protein

Fig. 5

Detection of the receptor binding ability of HN mutants. a The photographs of adsorbed cRBCs. After being incubated with 1% cRBCs solution at 4 °C for 30 min, the monolayers transfected with HN mutants were washed three times gently with cold PBS-CM. After washing the unbound cRBCs, the plate was observed under inverted microscope. The adsorbed cRBCs are indicated by arrows. (Magnification: 100 × ). b The plate same as a was treated with 50 mM NH4Cl at 4 °C to lyse bound cRBCs and the absorbance was read at 405 nm. c Different with a and b, the monolayers here were incubated with 1% cRBCs solution with zanamivir (2 mM) at 4 °C for 30 min. Then, the bound cRBCs were lysed and the absorbance was measured at 405 nm. (*, P < 0.05; **, P < 0.01; ***, P < 0.001; otherwise, P > 0.05)

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