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Fig. 3 | Virology Journal

Fig. 3

From: Roles of the highly conserved amino acids in the second receptor binding site of the Newcastle disease virus HN protein

Fig. 3

Quantitative detection results of cell surface expression efficiency of mutant HN proteins. Cell surface expression levels were quantified by FACS analysis. a Fluorescent histograms of site-directed mutants are shown. The x-axis indicates the fluorescence intensity value that is displayed on a logarithmic scale, and the y-axis shows cell counts. The numbers below the graph represent the percentage of cells in the M2 gate compared to the total number of cells. b Histogram of the average MFI of site-directed mutants. Data were normalized to the value obtained with wt HN, which was set at 100%. Values of the MFI are expressed as a percentage. The data are the average result of three independent experiments. Student’s t test was used to calculate the P value. c Fluorescent histograms of fragment deletion or replacement mutants. d Histogram of the average MFI of fragment deletion or replacement mutants. (**, P < 0.01; otherwise, P > 0.05)

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Virology Journal

ISSN: 1743-422X