Fig. 6

Morphogenesis dynamics of Orpheovirus particles. a TEM images at 24 h.p.i of asynchronous cycle of Orpheovirus evidencing the formation of VFs that presented particles in different maturation stages. b First discernible viral structures showing a crescent-shaped capsid precursor. c and d These structures extend and are filled by the internal content. e Recruitment of membranes in the periphery of the particles under assembly is also observed (red arrow). f Complete closure of the capsids and formation of mature particles. g and h Both by TEM and SEM images, it was evidenced that the mature particles have a flattening on one side (red arrow). i and j Orpheovirus one-step growth curve at an M.O.I. of 10