Fig. 1

Characterization of cytopathic effect of Orpheovirus. a V. vermiformis monolayer was infected by Orpheovirus using an M.O.I. of 10 and visualized by light microscopy. Cells became stretched with fusiform shapes at 3 h.p.i., and this effect became more evident at 9 h.p.i. and 12 h.p.i., respectively. At 12 h.p.i., fusiform branched cells were also observed. At 24 h.p.i., most cells observed were rounded; some fusiform cells were also visualized and cell lysis was more evident. b Counts of normal, fusiform (branched and not branched) and rounded cells. We observed a decrease of normal cells, while fusiform cells increased at 9 h.p.i., composing ~ 40% of the total infected cells. At 24 h.p.i., most cells were rounded, but some fusiform cells were also noticed (~ 20%)