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Fig. 6 | Virology Journal

Fig. 6

From: NAD-linked mechanisms of gene de-repression and a novel role for CtBP in persistent adenovirus infection of lymphocytes

Fig. 6

CtBP binding inhibitor, NSC95397, differentially impacts AdV gene expression across lymphocytic and epithelial cell lines. a) Western blot analysis of CtBP1 protein was performed as described in Materials and Methods on cell lysates collected with or without 24-h treatment with NSC95397. Both persistently-infected KE37 and BJAB were 66 dpi. b-e) RT-qPCR and analysis to assess viral gene expression performed as described for NSC95397 treatment in Material and Methods. b) Persistently-infected BJAB (≥ 61 dpi, n = 3), c) persistently-infected KE37 (≥ 80 dpi, n = 3), d) lytically-infected Jurkat (2 dpi, n = 3). Down-regulated values are shown as the negative reciprocal, which eliminates values falling between − 1 and 1 (indicated by shaded zone). Error bars show standard deviation on replicate wells. e) Lytically-infected epithelial cells A549 (2 dpi, error bars represent the SD of 3 independent experiments), CaLu1 (2 dpi, n = 1, SD of replicate wells), and Me-180 (2 dpi, n = 1, SD of replicate wells). f) Comparison of average change in E1A expression induced by NSC95397 treatment in T cell lines (KE37 and Jurkat, data shown in Fig. 6c and d) and epithelial cells (data shown in Fig. 6e). Shaded zone between − 1 and 1 as described above. Error bars show SD of fold-change values. P-value was determined using student’s t-test

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