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Fig. 3 | Virology Journal

Fig. 3

From: NAD-linked mechanisms of gene de-repression and a novel role for CtBP in persistent adenovirus infection of lymphocytes

Fig. 3

PMA and ionomycin treatment increase NAD+/NADH ratio in lymphocyte cell lines. a) Uninfected cells were treated with PMA/Iono for 4 h, and total cellular NAD+ and NADH nM concentrations were determined using a bioluminescent assay with standard curve. Numbers of replicates: BJAB - untreated, n = 8, treated, n = 3; Jurkat – untreated, n = 8, treated, n = 3; KE37 – untreated, n = 5, treated, n = 3. Fold-increase in treated over untreated is shown in bold in the gray bar (mean of the treated/mean of the untreated). Error bars show standard deviations of the NAD+/NADH ratios. P-values were determined using a student’s t-test. b) Impact of persistent infection on NAD+/NADH ratios. Total nM NAD+ and NADH were determined in persistently infected lymphocytes (> 50 dpi) as described in Material and Methods. For all samples, n = 5. Mean and standard deviation of the ratios is shown. P-values were calculated using student’s t-test

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