Fig. 1

Characterization of viral genome quantities and transcriptional repression in persistently-infected lymphocytes. a) Viral genome copy numbers determined by qPCR as previously described (5). BJAB, KE37, and Jurkat were infected with a MOI 50. Error bars show median value with range. Cell information: BJAB, persistently-infected (n = 9) and acutely-infected (n = 3); KE37, persistently-infected (n = 9) and acutely-infected (n = 3); lytically-infected Jurkat (n = 5). Persistently-infected lymphocytes were evaluated between 50 to 241 dpi, lytically-infected Jurkat cells were evaluated at 2 to 4 dpi. b) Viral transcription in persistently-infected lymphocytes was determined by RT-qPCR and relative amounts of mRNA calculated as described in Materials and Methods and normalized to the housekeeping gene EIF1 (which was not affected by infection, and was set to 1). The negative reciprocal was taken for values less than one to show down-regulation on the same scale. c & d) Relative amounts of viral transcripts E1A-13S, E3gp19K, and hexon in persistently-infected (c) and lytically-infected cells (d) were determined by RT-qPCR using equal amounts of RNA. Relative amounts were calculated as described in Material and Methods and then normalized to E1A (which was set to 1). Experiments were repeated at least 3 times with similar results. BJAB (n = 4, 53–60 dpi), KE37 (n = 4, 5–82 dpi), Jurkat (n = 3, 2–4 dpi), A549 (n = 3, 1–2 dpi)