Virus | RT- qPCR | Duplex real time RT-RAA | LFS RT-RAA | ||
---|---|---|---|---|---|
Positive | Negative | Positive | Negative | ||
EV71 | |||||
 Positive | 84 | 7 | 82 | 9 | |
 Negative | 1 | 353 | 1 | 353 | |
 Diagnostic sensitivity (%) | 92.3 |  | 90.1 |  | |
 Diagnostic specificity (%) |  | 99.7 |  | 99.7 | |
 PPV (%) | 98.8 |  | 98.8 |  | |
 NPV (%) |  | 98.1 |  | 97.5 | |
 Kappa | 0.943 |  | 0.929 |  | |
CA16 | |||||
 Positive | 97 | 1 | 93 | 5 | |
 Negative | 0 | 347 | 0 | 347 | |
 Diagnostic sensitivity (%) | 99.0 |  | 94.9 |  | |
 Diagnostic specificity (%) |  | 100 |  | 100 | |
 PPV (%) | 100 |  | 100 |  | |
 NPV (%) |  | 99.7 |  | 98.6 | |
 Kappa | 0.993 |  | 0.967 |  |