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Table 3 Comparison of clinical evaluation of two RT-RAA assays and RT-qPCR assay to detect EV71 and CA16

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

Virus RT- qPCR Duplex real time RT-RAA LFS RT-RAA
Positive Negative Positive Negative
EV71
 Positive 84 7 82 9
 Negative 1 353 1 353
 Diagnostic sensitivity (%) 92.3   90.1  
 Diagnostic specificity (%)   99.7   99.7
 PPV (%) 98.8   98.8  
 NPV (%)   98.1   97.5
 Kappa 0.943   0.929  
CA16
 Positive 97 1 93 5
 Negative 0 347 0 347
 Diagnostic sensitivity (%) 99.0   94.9  
 Diagnostic specificity (%)   100   100
 PPV (%) 100   100  
 NPV (%)   99.7   98.6
 Kappa 0.993   0.967  
  1. PPV Positive predictive value, NPV Negative predictive value