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Table 3 Comparison of clinical evaluation of two RT-RAA assays and RT-qPCR assay to detect EV71 and CA16

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

VirusRT- qPCRDuplex real time RT-RAALFS RT-RAA
PositiveNegativePositiveNegative
EV71
 Positive847829
 Negative13531353
 Diagnostic sensitivity (%)92.3 90.1 
 Diagnostic specificity (%) 99.7 99.7
 PPV (%)98.8 98.8 
 NPV (%) 98.1 97.5
 Kappa0.943 0.929 
CA16
 Positive971935
 Negative03470347
 Diagnostic sensitivity (%)99.0 94.9 
 Diagnostic specificity (%) 100 100
 PPV (%)100 100 
 NPV (%) 99.7 98.6
 Kappa0.993 0.967 
  1. PPV Positive predictive value, NPV Negative predictive value