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Table 2 Assay data used for calculating the detection limit of EV71 and CA16

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

Standard DNAa (copies/reaction)

EV71posb

CA16posb

Real time RAA

LFS RAA

Real time RAA

LFS RAA

104

8/8

8/8

8/8

8/8

103

8/8

8/8

8/8

8/8

100

8/8

8/8

8/8

8/8

20

5/8

0/8

6/8

0/8

10

3/8

0/8

4/8

0/8

1

0/8

0/8

0/8

0/8

  1. aTenfold serially diluted standard DNA
  2. bThe number of positive results per 8 reactions with diluted standard DNA