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Table 2 Assay data used for calculating the detection limit of EV71 and CA16

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

Standard DNAa (copies/reaction)EV71posbCA16posb
Real time RAALFS RAAReal time RAALFS RAA
1048/88/88/88/8
1038/88/88/88/8
1008/88/88/88/8
205/80/86/80/8
103/80/84/80/8
10/80/80/80/8
  1. aTenfold serially diluted standard DNA
  2. bThe number of positive results per 8 reactions with diluted standard DNA