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Fig. 4 | Virology Journal

Fig. 4

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

Fig. 4

Performance of EV71 LFS RT-RAA assay. a Optimization experiment of LFS RT-RAA reaction time. When the reaction time was longer than 20 min, the test line was visible. b Analytical sensitivity of the LFS RT- RAA assay. Lane1, 1 × 106 copies, Lane2, 1 × 105copies, Lane3, 1 × 104copies, Lane4, 1 × 103copies, Lane5, 1 × 102 copies, Lane6, 1 × 101 copies, Lane7, 1 copy, Lane8, no template control. c Analytical specificity of the LFS RT-RAA assay. Only EV71 specimen was amplified. The other samples were not amplified. Lane1, EV71, Lane2, CA 16, Lane3, CA6, Lane4, CA10, Lane5, CA5, Lane6, CA9, Lane7, CA24, Lane8, CB2, Lane9, CB4, Lane10, PV2, Lane11, PV3, Lane12, Eco30, Lane13, HEV14, Lane14, no template control

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Virology Journal

ISSN: 1743-422X