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Fig. 1 | Virology Journal

Fig. 1

From: Applicability of duplex real time and lateral flow strip reverse-transcription recombinase aided amplification assays for the detection of Enterovirus 71 and Coxsackievirus A16

Fig. 1

Detection of the RAA amplicons by lateral flow strip. The sample pad contains gold-labeled anti-FAM antibodies, the test line was coated with biotin-ligands, and control line was coated with anti-rabbit antibodies. The double-labeled amplicons (FAM and biotin) were diffused through the chromatographic membrane, and when they diffused to the test line, the products were captured by the biotin -ligands, resulting in an appearance of red-pink color. Non-captured particles will be fixed at the control line by anti-rabbit antibodies. In the absence of target amplicons, color will appear at control line only [26]

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