Evaluation of novel inactivated vaccines for the SAT 1, SAT 2 and SAT 3 serotypes of foot-and-mouth disease in pigs

Table 1 The primers used for PCR to replace the P1 genes of three serotypes in pO Manisa 3B3C (p3B3C) template

Serotype	Direction	Primer sequences
SAT1-BOT	Forward	5′-AAGGTCCAGAAAAGGCTCAAGGGAGCAGGCCAGTCGTCACCA-3’
SAT1-BOT	Reverse	5′-GAGCAGGTCAAAATTAGAAGCTGTTTGGCAGGTTTAACAAG-3’
SAT2-ZIM	Forward	5′-AACAAAGGTCCAGAAAAGGCTCAAGGGAGCGGGACAGTCATCA-3’
SAT2-ZIM	Reverse	5′-TTTGAGCAGGTCAAATTTAGAAGTGCACAGTTGTTTCTCGACG-3’
SAT3-ZIM	Forward	5′-AAACAAAGGTCAGAAAAGGCTCAAGGGAGCAGGCCAATCCTCCC-3’
SAT3-ZIM	Reverse	5′-TTTGAGCAGGTCAAAATTTAGAAGTTTGTTTGTCAGGTGCAACCA-3’

ISSN: 1743-422X