Table 1 Primers for BTV1 ten segments and BTV2 segment 2
From: PCR-based reverse genetics strategy for bluetongue virus recovery
Primer | Sequence (5′-3′) | Amplicon |
|---|---|---|
S1–1-20-F | TAATACGACTCACTATAGTTAAAATGCAATGGTCGCA | BTV1 segment 1 |
S1–3944-3925-R | GTAAGTGTAATGCGGCGCGT | |
S2–1-20-F | TAATACGACTCACTATAGTTAAAATAGTAGCGCGATG | BTV1 segment 2 |
S2–2940-2921-R | GTAAGTCTAATAGTGCGCGG | |
S3–1-20-F | TAATACGACTCACTATAGTTAAATTTCCGTAGCCATG | BTV1 segment 3 |
S3–2772-2753-R | GTAAGTGTGTTCCCGCTGCC | |
S4–1-20-F | TAATACGACTCACTATAGTTAAAACATGCCTGAGCCA | BTV1 segment 4 |
S4–1981–1962-R | GTAAGTTGTACATGCCCCCC | |
S5–1-20-F | TAATACGACTCACTATAGTTAAAAAAGTTCTCTAGTT | BTV1 segment 5 |
S5–1772–1753-R | GTAAGTTGAAAAGTTCTAGT | |
S6–1-20-F | TAATACGACTCACTATAGTTAAAAAGTGCGCCCTTAG | BTV1 segment 6 |
S6–1635–1616-R | GTAAGTGTAAGTGCTTCCCG | |
S7–1-20-F | TAATACGACTCACTATAGTTAAAAATCTATAGAGATG | BTV1 segment 7 |
S7–1156–1137-R | GTAAGTGTAATCTAAGAGAC | |
S8–1-20-F | TAATACGACTCACTATAGTTAAAAAATCCTTGAGTCA | BTV1 segment 8 |
S8–1125–1106-R | GTAAGTGTAAAATCCCCCCC | |
S9–1-20-F | TAATACGACTCACTATAGTTAAAAAATCGCATATGTC | BTV1 segment 9 |
S9–1049–1030-R | GTAAGTGTAAAATCGCCCTA | |
S10–1-20-F | TAATACGACTCACTATAGTTAAAAAGTGTCGCTGCCA | BTV1 segment 10 |
S10–822-803 | GTAAGTGTGTAGCGCCGCAT | |
rS2–1-20-F | TAATACGACTCACTATAGTTAAAACAGGATCGCGATG | BTV2 segment 2 |
rS2–2943-2924-R | GTAAGTTGAACAGATCGCGG |