Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression

Table 1 Blood samples used for epitope mapping

Animal no.	BoLA-DRB3 allele^a	BoLA-DQA1 allele^a	Breed	Age^b (months)	PBLs^c	Proviral load	Anti-BLV antibody (ELISA)^e
Animal no.	A:B	A:B	Breed	Age^b (months)	(cells/μl)	(CoCoMo-qPCR)^d	Anti-BLV antibody (ELISA)^e
Susceptible cattle
S4	1601:1601	10012:12021	JB^f	9	10,400	76,029	+
S2	1601:1601	10012:10012	JB	10	11,600	96,232	+
S6	1601:1601	10012:10012	JB	9	10,200	100,377	+
Resistant cattle
R1	2703:1501	10011:0204	JB	9	4210	263	+
Normal cattle
N1	0503:1501	10011:0101	JB	9	10,200	50,877	+

^aBoth alleles, A and B, for each animal are shown
^bAge at the time of blood sample collection
^cPeripheral blood lymphocyte (PBL) count per 1 μl of blood (measured at a single time point)
^dProviral load (expressed as the number of copies of provirus per 10⁵ peripheral blood mononuclear cells) was evaluated using the CoCoMo-qPCR-2 assay
^eEnzyme-linked immunosorbent assay was performed using the BLV ELISA kit. +, BLV-positive
^fJB, Japanese black

ISSN: 1743-422X