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Table 1 Swine-specific primer sequences used for quantitative SYBR ROX-1 real-time PCR

From: Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function

Gene source/accession no.

Accession number

Primer sequence (5′-3′)

Annealing temperature

(°C)

Products

(bp)

LMP7

AF059493

For: AGTGATTGTGGCGGTGGATT

56

328

Rev: CCGAGTCCCATTTTCATCCA

UBP

AF134195

For: GTGAGAACTGTGGCAGGAAGACC

58

347

Rev: TTCCCAGGACACCCAACAGA

MHC-I

AY135587

For: GAGGGGCAGGAGTATTGGGATAG

58

309

Rev: CCTCAATTGCTCCGCCACAT

Calreticulin

GQ984146

For: ATGACTGCTAGGTGTTTAAAATTA

56

226

Rev: GGATCTCTGGCAGGTCAAGT

β-actin

U 07786

For: TCATCACCATCGGCAACT

59

547

Rev: TTCCTGATGTCCACGTCGC

  1. Note: β-actin was used as the internal control
  2. bp: base pairs; for: forward primer; rev: reverse primer
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Virology Journal

ISSN: 1743-422X