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Fig. 5 | Virology Journal

Fig. 5

From: The tyrosine 73 and serine 83 dephosphorylation of H1N1 swine influenza virus NS1 protein attenuates virus replication and induces high levels of beta interferon

Fig. 5

RIG-I contributes to IFN-β production during infection with Y73F and S83A mutants. a 293 T cells were infected with rSIV or mutated virus at an MOI of 1 for 24 h, and the levels of RIG-I mRNA were investigated by real-time PCR. b 293 T cells were transfected with siRNA specific for RIG-I (100 nmol/ml) or control scrambled siRNA for 48 h and then inoculated with rSIV or mutated virus at an MOI of 1. The cells were harvested at 24 hpi. The protein levels were determined using an anti-RIG-I antibody, and β-actin was used as the protein loading control. c 293 T cells transfected with RIG-I-specific siRNA were infected with rSIV or mutated viruses at an MOI of 1 for 24 h and then harvested for determination of IFN-β and ISG56 mRNA levels by real-time PCR

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Virology Journal

ISSN: 1743-422X