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Fig. 3 | Virology Journal

Fig. 3

From: The tyrosine 73 and serine 83 dephosphorylation of H1N1 swine influenza virus NS1 protein attenuates virus replication and induces high levels of beta interferon

Fig. 3

NS1 protein localization is not affected by Y73F or S83A dephosphorylation. MDCK cells were infected with rSIV and mutated viruses at an MOI of 1. At 6 hpi, the cells were fixed and stained with a specific anti-NS1 polyclonal antibody and a Alexa Fluor 488–labeled secondary antibody (green). Nuclei were stained with DAPI (blue). The localizations of NS1 proteins were visualized using a Carl Zeiss fluorescence microscope. Merge: merged image of double immunofluorescence staining of the same field

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Virology Journal

ISSN: 1743-422X