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Fig. 2 | Virology Journal

Fig. 2

From: The tyrosine 73 and serine 83 dephosphorylation of H1N1 swine influenza virus NS1 protein attenuates virus replication and induces high levels of beta interferon

Fig. 2

Growth kinetics of recombinant viruses with NS1 mutants lacking a phosphorylation site. a Growth kinetics of the recombinant viruses in MDCK cells. Recombinant viruses possessing wild-type NS1, NS1 Y73F and NS1 S83A were generated by reverse genetics, as described in the Materials and Methods. MDCK cells were infected with rSIV or mutated virus (rSIV NS1 Y73F and rSIV NS1 S83A) at an MOI of 0.001. Supernatants were collected at 12, 24, 48, and 72 hpi, and virus titers were determined by TCID50 assay. The mean values from three independent experiments are shown for each sample. b Expression kinetics of NS1 protein during infection. MDCK cells on 6-well plates were infected at an MOI of 0.001 with rSIV or mutated viruses, and cell lysates were collected at the indicated time points for western blot analysis of NS1 protein levels.C. Percentage of the NS1 protein reduction level. The percentage of protein reduction (%) was calculated by the formula [(NS1 expression of rSIV infected cells - NS1 expression of mutant viruses infected cells)/ NS1 expression of rSIV infected cells] × 100%; Abscissa: time points after infection

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Virology Journal

ISSN: 1743-422X