Table 1 Primers for analysis of gene expression by qRT-PCR
Primers | Sequence |
|---|---|
RT-miR-146b-5p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAACGCCTA |
RT-miR-125b-5p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCACAAGTT |
RT-miR-30a-5p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGCTTCCA |
RT-miR-27b-3p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCAGAACT |
RT-miR-16c-5p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTCCAGTA |
RT-miR-130b-3p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACGCCCTT |
miR-146b-5p (F) | GCCGTGAGAACTGAATTCCATA |
miR-125b-5p (F) | GCCGTCCCTGAGACCCTAA |
miR-30a-5p (F) | GCCGTGTAAACATCCTTGACTG |
miR-27b-3p (F) | GCCGTTCACAGTGGCTAAG |
miR-16c-5p (F) | GCCGTAGCAGCACGTAAATA |
miR-130b-3p (F) | GCCGCAGTGCAATAATGAAA |
UR-primer | CAGTGCGTGTCGTGGAGT |
U6 (F) | CTCGCTTCGGCAGCACA |
U6 (R) | GCGTGTCATCCTTGCGC |
Beclin-1 (F) | AAGAGGTGCCTGGAGATCCT |
Beclin-1 (R) | CGTCCTCCAGCTCCTGAATC |
β-Actin (F) | CCGGGCATCGCTGACA |
β-Actin (R) | GGATTCATCATACTCCTGCTTTGCT |