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Fig. 3 | Virology Journal

Fig. 3

From: Downregulation of microRNA-30a-5p contributes to the replication of duck enteritis virus by regulating Beclin-1-mediated autophagy

Fig. 3

miR-30a-5p overexpression suppresses Beclin-1 expression and DEV-induced autophagy. a DEF cells were pretreated with control (without), mimic-NC and miR-30a-5p mimic for 4 h and then infected with DEV. Cells were lysed and blotted with antibodies against Beclin-1, p62, LC3, β-actin and UL41 at 36 hpi. b The optical densities of each protein band from (a) were measured by densitometric scanning, and the optical density ratios of Beclin-1/β-actin, P62/β-actin LC3II/I, and UL41/β-actin were calculated. c DEF cells were pretreated with control (without), inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV. Cells were lysed and blotted with antibodies against Beclin-1, p62, LC3, β-actin and UL41 at 36 hpi. d The optical densities of each protein band from (c) were measured by densitometric scanning, and the optical density ratios of Beclin-1/β-actin, P62/β-actin LC3II/I, and UL41/β-actin were calculated. The data are presented as the mean ± SD for three independent experiments. *p < 0.05; **p < 0.01, compared with the control group

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