Fig. 2

Luciferase reporter assay for the interaction between miR-30a-5p and Beclin-1 mRNA. a The seed sequence of miR-30a-5p and its target site in the 3’UTR of Beclin-1 mRNA are shown in red, and eight nucleotides were mutated in the 3’UTR of Beclin-1 mRNA (underlined). b Activity of the luciferase gene linked to the 3’UTR of Beclin-1 mRNA. Wild-type pmirGLO-WT-Beclin-1 (WT-Beclin-1) or mutant pmirGLO-MU-Beclin-1 (MU-Beclin-1) was transfected into COS7 cells with the miR-30a-5p mimic or the negative control (miR-NC). Luciferase activity was measured after 36 h. c DEF cells were pretreated with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV for 36 h. The expression level of miR-30a-5p was assessed by qRT-PCR. d DEF cells were pretreated with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV for 36 h. The expression level of Beclin-1 was assessed by qRT-PCR. The data are presented as the mean ± SD for three independent experiments. *p < 0.05; **p < 0.01, compared with the control group (miR-30a-5p + WT-Beclin-1)