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Fig. 1 | Virology Journal

Fig. 1

From: Downregulation of microRNA-30a-5p contributes to the replication of duck enteritis virus by regulating Beclin-1-mediated autophagy

Fig. 1

Expression levels of miRNAs and Beclin-1 mRNA after DEV infection of DEF cells. a Expression levels of 6 DEF-encoded miRNAs were detected using stem-loop qRT-PCR at 36 hpi. b Expression of miR-30a-5p was measured by stem-loop qRT-PCR at the indicated time points. U6 was used as an endogenous control. c Expression of miR-16c-5p was measured by stem-loop qRT-PCR at the indicated time points. U6 was used as an endogenous control. d Expression of Beclin-1 mRNA was measured by qRT-PCR at the indicated time points. β-Actin was used as an endogenous control. The data are presented as the mean ± SD for three independent experiments. *p < 0.05; **p < 0.01

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Virology Journal

ISSN: 1743-422X