Fig. 5

HDAC1 silencing decreases HIV-1 late reverse transcription. HeLa-CD4-LTR-β-gal cells were transfected with 15 nM siHDAC1 or control siRNA and infected with DNase I-treated HIV-1 NL4–3 virions (10 ng p24 per 8 × 10⁴ cells) 48 h after transfection. At different time point post-infection, cells were harvested and DNA was extracted. Late RT transcripts (6–12 h) (a) and integrated viral DNA (48 h) (b) were quantified by real-time PCR, as described in Materials and Methods. Results are expressed as percentage of the control (siControl-transfected cells are set as 100%). Data represent means ± SD of triplicates from a single experiment (n = 3) and were analysed by the Student t test (**, p < 0.01, ****, p < 0.0001). Data are representative of two independent experiments