Fig. 4

HDAC1 overexpression increases HIV-1 replication. a HeLa-CD4-LTR-β-gal cells were transfected with the pFLAG-HDAC1 expression vector or the pBact-FLAG empty vector (375 ng) and 48 h later infected with HIV-1 NL4–3 virions (10 ng p24 per 8 × 10⁴ cells). Supernatants were harvested at 48 h post-infection and measured for virus levels through p24 ELISA assay. Results are expressed as percentage of the control and represent means ± SD of triplicates from a single experiment (n = 3). Data are representative of two independent experiments and were analysed by the Student t test (*, p < 0.05). b Total cell lysates (48 h post-infection) was analyzed by Western blot with anti-FLAG, anti-HDAC1 and anti-β-actin antibodies