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Fig. 3 | Virology Journal

Fig. 3

From: Histone deacetylase 1 interacts with HIV-1 Integrase and modulates viral replication

Fig. 3

HDAC1 knockdown inhibits HIV-1 replication. a HeLa-CD4-LTR-β-gal cells were transfected with 15 nM siHDAC1 (Hs_HDAC1_5) or control siRNA (GFP-22 siRNA) and, 48 h after transfection, were infected with NL4–3 HIV-1 virions (10 ng p24 per 8 × 104 cells). Culture supernatants were harvested at different time points post-infection and viral levels were measured through p24 ELISA assay. b, c siRNA-transfected HeLa-CD4-LTR-β-gal cells were harvested at indicated time points and analyzed for endogenous HDAC1 expression by quantitative RT-PCR (B) and Western blot (c). d Cell viability was determined at day 1, 2 and 3 after siRNA transfection, using the MTS/PMS assay. Results are expressed as percentage of the data obtained with control siRNA-transfected cells. Data in A, B, and D represent means ± SD of triplicates from a single experiment (n = 3). Results are representative of two independent experiments and were analysed by the Student t test (*, p < 0.05, **, p < 0.01 ***, p < 0.001, ****, p < 0.0001)

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