Fig. 2
From: Histone deacetylase 1 interacts with HIV-1 Integrase and modulates viral replication
HDAC1 specifically interacts with HIV-1 integrase. a 293 T-INsala cells (stably expressing HIV-1 IN) were transfected with pFLAG-HDAC1. Cell lysates were then subjected to immunoprecipitation with anti-IN (α-IN), anti-FLAG (α-FLAG) or control IgG antibodies. Immunoprecipitates and total cell extracts (input) were analyzed by Western blot with anti-IN or anti-FLAG antibodies. b Purified IN was incubated with purified 6xHis-tagged HDAC1 or with Ni-NTA beads alone (negative control). Bound proteins and input were analyzed by Western blot using anti-6xHis or anti-IN antibodies