Fig. 1From: Heparin sulfate is the attachment factor of duck Tembus virus on both BHK21 and DEF cellsEffects of desulfation on DTMUV attachment. BHK21 cells were treated with different concentrations of sodium chlorate (a) for 1 week and then the cells were used for DTMUV binding assays. BHK21 cells were treated with 20 mM sodium chlorate for 1 week in the presence or absence of 20 mM sodium chlorate or 4 mM sodium sulfate (b) in RPMI medium containing 10% FBS and then the cells were used for DTMUV binding assays. c After treating the cells with sodium chlorate or sodium sulfate, the cell cytotoxicity was tested using a CCK8 kit. The data shown here the average and standard error of four independent experiments. The results of each treatment were compared to their untreated control by Student’s t test, *P<0.05, **P<0.01, and ***P<0.001, ns means not significantBack to article page