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Fig. 1 | Virology Journal

Fig. 1

From: Heparin sulfate is the attachment factor of duck Tembus virus on both BHK21 and DEF cells

Fig. 1

Effects of desulfation on DTMUV attachment. BHK21 cells were treated with different concentrations of sodium chlorate (a) for 1 week and then the cells were used for DTMUV binding assays. BHK21 cells were treated with 20 mM sodium chlorate for 1 week in the presence or absence of 20 mM sodium chlorate or 4 mM sodium sulfate (b) in RPMI medium containing 10% FBS and then the cells were used for DTMUV binding assays. c After treating the cells with sodium chlorate or sodium sulfate, the cell cytotoxicity was tested using a CCK8 kit. The data shown here the average and standard error of four independent experiments. The results of each treatment were compared to their untreated control by Student’s t test, *P<0.05, **P<0.01, and ***P<0.001, ns means not significant

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