Fig. 3

Identification of human pegivirus (HPgV) infection by Revese transcriptase polymerase chain reaction (RT-PCR). Plasma samples before (lane a) and after (lane b) viral treatment with daclatasvir and sofosbuvir. Viral RNA was extracted using viral RNA extraction kit followed by synthesis the complementary DNA (cDNA) with a high-capacity cDNA kit (Applied Biosystems). The PCR reaction targeting the 5′ UTR was performed in the thermal cycle, for 40 cycles to amplify a 173 bp fragment. PCR product was loaded on 2% gel and electrophoresed for 20 min at 120 V and 90 A after that the gel was visualized and imaging using photo-documentation system (Biometria). Lane c is a negative control, and lane d contains a DNA marker (50 bp ladder)