Skip to main content
Fig. 5 | Virology Journal

Fig. 5

From: The 164 K, 165 K, and 167 K residues of VP1 are vital for goose parvovirus proliferation in GEFs based on PCR-based reverse genetics system

Fig. 5

The 164 K, 165 K, and 167 K are vital for GPV proliferation. a Construction and transfection of pIRC16 K164A, pIRC16 K165A, pIRC16 K167A, pIRC16 AAPA and pIRC16 KKRK. On one hand, the cells were collected to detect the expression level of GPV Rep by IFA and WB at 72 h post-transfection. F0 represents the virus transfected pIRC16 K164A, pIRC16 K165A, pIRC16 K167A, pIRC16 AAPA, and pIRC16 KKRK respectively. On the other hand, the cells were collected to infect GEFs at 72 h post-transfection. F1 represents the virus from passaged F0. b and (d) At 72 h post-transfection, the GPV Rep of F0 was detected by IFA and WB using the rabbit anti-GPV Rep polyclonal antibody. c and (e) At 72 h PI, the GPV Rep of F1 was detected by IFA and WB using the rabbit anti-GPV Rep polyclonal antibody

Back to article page
\